Plasmid_Backbone

Part:BBa_K5109060

Designed by: Lisa Faccincani   Group: iGEM24_Uni-Padua-IT   (2024-09-28)


pJUMP29-1A ∆NdeI

This part is an improvement of part BBa_J428341 that modifies a single base in the sequence, removing an undesired NdeI site. Mutation of the site is obtained via PCR with mutagenic primers. In order to do so, we performed a mutagenic PCR that changed the backbone sequence through mutagenic primers.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 47
    Illegal suffix found at 489
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 47
    Illegal NheI site found at 100
    Illegal NheI site found at 123
    Illegal SpeI site found at 490
    Illegal PstI site found at 504
    Illegal NotI site found at 53
    Illegal NotI site found at 497
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 47
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 47
    Illegal suffix found at 490
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 47
    Plasmid lacks a suffix.
    Illegal XbaI site found at 62
    Illegal SpeI site found at 490
    Illegal PstI site found at 504
    Illegal NgoMIV site found at 1863
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 467
    Illegal BsaI.rc site found at 84
    Illegal SapI.rc site found at 2661


[edit]
Categories
Parameters
None